normal rabbit igg control  (R&D Systems)

Journal: Chemical Science

Article Title: A (poly)Pro tip for preserving native disulfide connectivity during thiol–maleimide bioconjugation of disulfide-rich peptides

doi: 10.1039/d5sc08821f

Figure Lengend Snippet: Crystal structure of tetrameric ectodomain of LINGO-1 (left). Structure of the LINGO-1 N-cap [1–20] region, displaying a DRP motif (top right, cornflower blue). 3D NMR solution structure obtained for synthetic LINGO-1[1–20], 1 (box, bottom right, magenta).

Article Snippet: Remarkably, our polyclonal IgG detected LINGO-1 at a five-fold higher dilution than the commercial polyclonal IgG antibody AF3086 In a previous study, screening of all commercially available IgGs identified polyclonal IgG AF3086 as the most suitable for co-immunoprecipitation studies. (R&D Systems), while also yielding stronger overall staining intensity (Fig. S52 and S53).

Techniques:

Journal: Chemical Science

Article Title: A (poly)Pro tip for preserving native disulfide connectivity during thiol–maleimide bioconjugation of disulfide-rich peptides

doi: 10.1039/d5sc08821f

Figure Lengend Snippet: Synthesis of C-terminal amidated LINGO-1[1–20]-Gly-Anl 4. Reagents and conditions: [a] DMSO/H 2 O – 1 : 9, peptide conc. 0.32 mM*, 100 h, r.t.; [b] AcOH/160 mM aq. HCl – 93.6 : 6.4 containing 62.5 mM of I 2 , peptide conc. 1 mM*, 2 h, r.t. Abbreviations: Anl = 6-Azido- l -norleucine, represented in the scheme as “K(N 3 )”; DMSO = dimethyl sulfoxide; r.t. = room temperature. *Steps [a] and [b] were carried out on crude material, with concentrations calculated from the crude mass as if it corresponded to the pure compound.

Article Snippet: Remarkably, our polyclonal IgG detected LINGO-1 at a five-fold higher dilution than the commercial polyclonal IgG antibody AF3086 In a previous study, screening of all commercially available IgGs identified polyclonal IgG AF3086 as the most suitable for co-immunoprecipitation studies. (R&D Systems), while also yielding stronger overall staining intensity (Fig. S52 and S53).

Techniques:

Journal: Chemical Science

Article Title: A (poly)Pro tip for preserving native disulfide connectivity during thiol–maleimide bioconjugation of disulfide-rich peptides

doi: 10.1039/d5sc08821f

Figure Lengend Snippet: (A) ELISA cell assay showing the interaction between decreasing concentrations of polyclonal IgG and HEK cells expressing LINGO-1. Absorbance values at 450 nm were background-corrected by subtracting the signal from non-transfected HEK cells. (B) Superposition of the [1–20] region for LINGO-1-to-4 homologs shows that all Cys residues are conserved; additional conserved residues are highlighted in dark grey, whereas similar residues—defined by comparable hydrophilicity ( e.g. , Ser vs. Thr) or charge ( e.g. , Lys, Arg, or His)—are shown in light grey. (C) Western blot detail in which polyclonal IgG was used for staining HEK cells and LINGO homologs 1–4. Anti-GAPDH staining is shown at the bottom as a loading control.

Article Snippet: Remarkably, our polyclonal IgG detected LINGO-1 at a five-fold higher dilution than the commercial polyclonal IgG antibody AF3086 In a previous study, screening of all commercially available IgGs identified polyclonal IgG AF3086 as the most suitable for co-immunoprecipitation studies. (R&D Systems), while also yielding stronger overall staining intensity (Fig. S52 and S53).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Transfection, Western Blot, Staining, Control